Beckman Coulter, Inc., Brea, CA, Advanced Technologies Center, Chemistry, 11/91 - Present
Senior Staff Scientist (8/00 - Present); Project Leader (4/98 - 8/00); Staff Scientist (5/96 - 4/98); Principal Scientist (3/94 - 5/96); Senior Scientist (11/91 - 3/94)

Responsibilities included the following:

• R & D in organic chemistry, near infrared fluorescent dye chemistry, immuno chemistry, nucleoside and nucleotide chemistry.
  
• Designed and synthesized fluorescent dyes and encoding these dyes into polymer particles. These particles are used in the Beckman MiniFlow system for many applications including SNP, cytokines immunoassay and others.
  
• Designed and synthesized fluorescent dyes for reticulocytes staining applications.
  
• Synthesized derivatives of anticonvulsant drugs, e.g., phenobarbital, phenytoin, valproic acid, primidone, carbamazepine, ethosuximide; and antiarrhythmic drugs, e.g. quinidine; and their corresponding biotin derivatives for conjugation with avidin or avidin coated latex particles, and applied these conjugates to the therapeutical drugs monitoring (TDM) assay using Beckman clinical diagnostic instruments.
  
• Synthesized highly sulfated alpha, beta, and gamma-cyclodextrins (HS-CDs) as a new class of chiral selectors for separation of enantiomeric pairs of drugs by capillary electrophoresis.
  
• Designed and synthesized four near infrared dye labeled 2',3'-dideoxynucleoside triphosphates (ddNTP-fluor) for DNA sequencing project. These dye-terminators reagents are used in the Beckman CEQ system
  
• Designed and synthesized high molecular weight polyacrylamide gel for application in capillary electrophoresis (CE) DNA sequencing.
  
• Designed and synthesized fluorescent dyes for CE application in assays of polysaccharides, monosaccharides, and amino acids.
  
• Synthesized many DNA intercalating agents, e.g. ethidium bromide derivatives, TOTO, YOYO.
  
• Prepared inverse amidites, 3'-O-DMT-5'-phosphoramidite-2'-deoxynucleosides, for the synthesis of a new class of antisense oligonucleotides (INV-oligonucleotides).
  
• Planning, designing and conducting the synthesis of hydrophilic-nanostructures (e.g., dendrimers) and investigating the various applications of the architecture controlled synthetic macromolecules.
  
• Studied the structure-activity relationships (SAR) of the cyanine dyes using molecular modeling workstation (MSI, QUANTA/CHARMm).
  
• Supervisory responsibility.
  
• Project management: Lead the Specialty Chemical Operations (SCO) project to supply and support Beckman Technical Operations and Manufacturing groups with drug bidentates, dye-terminators, HS-CDs, APTS, polyacrylamide gels and other compounds. These specialty chemicals generated more than million/year in sales.
  
• Chemical Hygiene Officer, Safety Officer and laboratory safety regulation and auditing.
  
• Mentor, Young Scholars Program (National Science Foundation).
  

• R & D in medicinal chemistry, drug discovery program.
  
• Supervised a group of chemists conducting the cGMP synthesis of bulk pharmaceuticals as anti-AIDS, anti-viral, and anti-cancer drugs for different phases of clinical trials under contract by the National Cancer Institute.
  
• Responsible for all administrative aspects of the contract.
  
• Carried the synthesis and process development from small-scale to multikilogram-scale of anti-AIDS drug.
  
• Synthesized many anti-AIDS drugs for different phases of clinical trials, which include: dideoxynucleosides (ddN's), fluoroarabinofuranose, tetrazolium salt, thiazolo derivative, pentamidine, and several biologically active dyes in compliance with cGMP's.
  
• Thorough knowledge of nucleoside, carbohydrate and heterocyclic chemistry.
  
• Used enzymatic catalysis in preparative organic synthesis.
  
• Working knowledge of SOPs, QCs, cGMP's as well as OSHA and EPA regulations.
  

• Research in medicinal chemistry and nucleoside chemistry.
  
• Designed and synthesized many nucleosides and C-nucleosides as anti-viral and anti-cancer agents.
  
• Defined the unambiguous synthetic routes for the syntheses of all three N-methyl derivatives of C-Ribavirin.
  
• Synthesized certain N-2 and N-4 substituted analogues of C-Ribavirin.
  
• Studied the ring-size effect on the biological activity of adenosine analogues.
  
• Studied the 5,5-ring system analogues of purine nucleoside.
  
• Knowledge in heterocyclic chemistry, drugs design, and structure-activity relationships.
  

• Research in bio-organic chemistry.
  
• Designed and synthesized vitamin D analogues as cancer chemopreventive and therapeutical agents, suicide inhibitors, and ligands for affinity chromatography.
  
• Synthesized A-homo and A-nor oxa analogues of the most potent biologically active metabolite of vitamin D3, 1a,25-(OH)2-vitamin D3.
  
• Investigated the ring size effect on the equilibrium ratio between vitamin D and previtamin D.
  
• Synthesized eneynlactone compounds by using the flash vacuum pyrolysis (FVP) technique.
  
• Studied the C-C bond coupling reaction using organopalladium compound as catalyst.
  

• Research in organic chemistry.
  
• Studied the thermal rearrangement of vinylallenes.
  
• Synthesized and characterized 19-substituted 11-cis-vitamin A analogues.
  
• Detailed studies on the thermal pericyclic reaction of geometric isomers of vitamin A analogues.
  
• Investigated the solution conformations of retinoid analogues using Nuclear Overhauser Effects (NOE) technique.
  
• Studied the synthetic approaches to geometrically defined conjugated trienes.
  
• Carried out the mechanistic studies of the [1,5]-sigmatropic rearrangement of vinylallenes; kinetic and stereochemical investigations of substituent effects on these pericyclic processes.
  
• Carried out a Temperature Dependent Kinetic Isotope Effect (TDKIE) study for the [1,5]-sigmatropic rearrangement of vinylallenes.
  
• Developed a computer program to analyze the experimental data.
  

• Synthesized certain organosilicon compounds as plant growth-regulating agents.

• Dissertation: "Sulfoxide Induced Acceleration and Enhancement of Geometric Selectivity of [1,5]-Sigmatropic Hydrogen Shifts of Vinylallenes and Studies on a Vinylallene Mediated Synthesis of 19-Substituted Retinols".
  
• Research Director: Professor William H. Okamura

• Research Director: Professor William H. Okamura
  

• Research Director: Professor Sheng-Lieh Liu

• Extensive experience in all aspects of synthetic and analytical organic chemistry.
  
• Hands-on experience in the operational and interpretational aspects of FT-NMR (13C, 1H), FT-IR, UV, capillary electrophoresis, and Visible spectroscopy and the interpretation of mass spectra.
  
• Thorough knowledge of analytical and preparative HPLC, medium pressure LC, Chromatotron LC, GC, CE and other forms of chromatography.
  
• Experience with inert atmosphere techniques, low temperature techniques, high vacuum systems.
  
• Computer programming. Computer modeling.
  
• Considerable familiarity with biochemistry, medicinal chemistry, immunology and ability to interact well within interdisciplinary group.
  

• Team Achievement Award, Beckman Instruments, Inc., 1997.
• Biographee, Marquis Who's Who in in the World, 18th Edition, 2001.
• Biographee, Marquis Who's Who in the West, Silver Anniversary 25th Edition, 1996~1997.
• Biographee, Marquis Who's Who in Science and Engineering, Who's Who in America, 1994~2003.
• Recipient, Phi Beta Kappa Alumni Award, UCR, 1984
• Recipient, Chancellor's Patent Fund Grant, UCR, 1984
• Recipient of three other scholarships, NTU, 1975 - 1979

• American Chemical Society (ACS)
  • Division of Organic Chemistry, ACS
  • Division of Chinese American Chemical Society, ACS
  • Division of Polymer Chemistry, ACS
• American Association for the Advancement of Science (AAAS)
  
• President, Confucius Chinese Language School, Walnut, California
  

1. Gupta, R.; Lee, S.Y.; Shen, Gene G.-Y.; Szydlo, S.; Deka, C. “Dyes And Methods of Detection of Nucleic Acid in Immature Red Blood Cells,” USA Patent Number 6,551,831, April 22, 2003
   
2. Shen, Gene G.-Y.; Oh, C. S.; Yancey, S. D.; Adams, C. W. “Synthesis of Chlorophenol Red Glucuronic Acid,” USA Patent Number 6,534,637, March 18, 2003.
   
3. Deka, C.; Lee, S.Y.; Shen, Gene G.-Y.; Szydlo, S.; Tsay, T.-T.; Gupta, R. "Dyes And Methods of Reticulocyte Enumeration,” USA Patent Number 6,368,864, April 9, 2002.
   
4. Chen, F-T A.; Evangelista, R. A.; Shen, Gene G.-Y.; Oh, C. S. “Chiral Separation of Pharmaceutical Compounds with Charged Cyclodextrins Using Capillary Electrophoresis,” USA Patent Number 6,316,613, November 13, 2001.
   
5. Shen, Gene G.-Y.; Dobashi, Thomas S. “Cyanine Dye Activating Group with Improved Coupling Selectivity,” USA Patent Number 6,002,003, December 14, 1999.

1. Chen, F.-T. A.; Shen, G. G.-Y.; Evangelista, R. A. “Characterization of Highly Sulfated Cyclodextrins”, J. of Chromatography A., 2001, 924, 523.
2. Shen, G.-Y.; Robins, R.K.; Revankar, G.R. "Synthetic Studies on the Isomeric N-Methyl Derivatives of C-Ribavirin", Nucleosides & Nucleotides 1991, 10(8), 1707.
3. Enas, J.D.; Shen, G.-Y.; Okamura, W.H. "A-Nor Vitamin D Analogs: Ring Size and Substituent Effects on the Previtamin D-Vitamin D Equilibrium", J. Am. Chem. Soc. 1991, 113, 3873.
4. Henry, H.L.; Fried, S.; Shen, G.-Y.; Barrack, S.A.; Okamura, W.H. "Effect of Three A-Ring Analogs of 1a,25-Dihydroxyvitamin D3 on 25-OH-D3-1a-Hydroxylase in Isolated Mitochondria and on 25-Hydroxyvitamin D3 Metabolism in Cultured Kidney Cells", J. Steroid Biochem. Molec. Biol. 1991, 38, 775.
5. Okamura, W.H.; Shen, G.-Y.; Barrack, S.A.; Henry, H.L. "Vitamin D Analogues: Potential inhibitors of Vitamin D Metabolism" In Vitamin D: Molecular, Cellular, and Clinical Endocrinology, Edited by Norman, A.W.; Schaefer, K.; Grigoleit, H.-G.; and Herrath, D., Walter de Gruyter and Co., Berlin, 1988, p 12-21.
6. Shen, G.-Y.; de Lera, A.R.; Norman, T.C.; Haces, A.; and Okamura, W.H. "Thermal Rearrangement of tert-Butyl Substituted 9,10- and 11,12-Allenic Retinoids: 11-cis-Isomers of 19,19,19- and 20,20,20-Trimethylretinoids", Tetrahedron Lett. 1987, 28, 2917.
7. Shen, G.-Y; Tapia, R.; and Okamura, W.H. "Substituent Effect Studies on the Thermal [1,5]-Sigmatropic Hydrogen Shifts of Vinylallenes", J. Am. Chem. Soc. 1987, 109, 7499.
8. Okamura, W.H.; Shen, G.-Y.; Tapia, R. "Sulfoxide Induced Acceleration and Enhancement of Geometric Selectivity of a [1,5]-Sigmatropic Hydrogen Shift", J. Am. Chem. Soc. 1986, 108, 5018.